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ecis zθ system  (Applied BioPhysics)


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    Structured Review

    Applied BioPhysics ecis zθ system
    A) hCMEC barrier resistance was assessed by <t>ECIS</t> <t>Zθ</t> to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001
    Ecis Zθ System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecis+z%CE%B8/ECIS+Z+Theta/bio_rxiv__64898__2026__03__16__711716-163-7-10
    Average 96 stars, based on 636 article reviews
    ecis zθ system - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models"

    Article Title: Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models

    Journal: bioRxiv

    doi: 10.64898/2026.03.16.711716

    A) hCMEC barrier resistance was assessed by ECIS Zθ to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001
    Figure Legend Snippet: A) hCMEC barrier resistance was assessed by ECIS Zθ to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001

    Techniques Used: Western Blot

    CA-VB KO in hCMEC protects from Aβ-induced apoptosis and BBB permeability . A) WB confirmed CA-VB absence in KO cells. B) DNA fragmentation, plotted as fold of change (FOC) of untreated control (ctrl) cells, measured by cell death ELISA. C) Representative IF images of active caspase-3/7 (green) and quantification to the right in CMEC treated with 25µM Q22 for 24 hours. Original magnification: 10x . D) IF of CA-VB KO vs WT hCMEC to detect mitochondrial membrane potential (Mito-tracker, red) as well as cytochrome C (Cyto C, green), in hCMEC challenged with Q22 for 16 hours. Zoom images of the merge signal at the bottom depict altered mitochondrial network with perinuclear mitochondria in Q22-treated hCMEC, but not in CA-VB KO cells. Original magnification:100x E) Mitochondrial H 2 O 2 measured with Amplex Red kit. F) Barrier resistance was measured over time with ECIS-Zθ in WT and CA-VB KO hCMEC treated or not with 10µM Q22. Data represents 3 individual experiments with 2 replicates each, graphed as mean ± SEM. Statistical significance was evaluated by Two-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01 ****P<0.0001
    Figure Legend Snippet: CA-VB KO in hCMEC protects from Aβ-induced apoptosis and BBB permeability . A) WB confirmed CA-VB absence in KO cells. B) DNA fragmentation, plotted as fold of change (FOC) of untreated control (ctrl) cells, measured by cell death ELISA. C) Representative IF images of active caspase-3/7 (green) and quantification to the right in CMEC treated with 25µM Q22 for 24 hours. Original magnification: 10x . D) IF of CA-VB KO vs WT hCMEC to detect mitochondrial membrane potential (Mito-tracker, red) as well as cytochrome C (Cyto C, green), in hCMEC challenged with Q22 for 16 hours. Zoom images of the merge signal at the bottom depict altered mitochondrial network with perinuclear mitochondria in Q22-treated hCMEC, but not in CA-VB KO cells. Original magnification:100x E) Mitochondrial H 2 O 2 measured with Amplex Red kit. F) Barrier resistance was measured over time with ECIS-Zθ in WT and CA-VB KO hCMEC treated or not with 10µM Q22. Data represents 3 individual experiments with 2 replicates each, graphed as mean ± SEM. Statistical significance was evaluated by Two-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01 ****P<0.0001

    Techniques Used: Permeability, Control, Enzyme-linked Immunosorbent Assay, Membrane

    Related Articles

    Electric Cell-substrate Impedance Sensing:

    Article Title: A neurotensin receptor type 1-derived pepducin acts as a biased allosteric modulator to regulate target receptor function
    Article Snippet: .. The biophysical parameters of the cell monolayer (i.e., electrical resistance to a 4000-Hz single frequency AC current) were recorded with an ECIS Zθ linked to a 96-well array station (Applied Biophysics, Troy, NY, USA) for a minimum period of 60 min prior to compound stimulation, to acquire a stable baseline. ..

    Article Title: Bidirectional Hypoxic Extracellular Vesicle Signaling Between Müller Glia and Retinal Pigment Epithelium Regulates Retinal Metabolism and Barrier Function
    Article Snippet: .. The electric cell-substrate impedance sensing [ECIS ® Zθ (theta)] instrument (Applied Biophysics Inc., Troy, NY, USA) was used to measure the normalized transcellular electrical resistance (TER) of the RPE as an indicator of the barrier integrity. .. Briefly, electrode arrays (96-wells, Applied Biophysics Inc., Catalog # 96W20idf PET) coated with 0.02% gelatine and 100 μM of cysteine were used to culture RPE.

    Article Title: Regenerating islet derived Family Member 4 (REG4) does not influence parameters of hemostasis in colorectal cancer.
    Article Snippet: .. Endothelial cell layer integrity was measured every 350 s at multiple frequencies using ECIS Zθ (Applied Biophysics, US) at 37 ◦C and 5 % CO2. .. After 24 h, when the endothelial barrier was formed, 75 % of the medium was replaced by supernatant and the resistance was measured for at least 48 h. Barrier function graphics for different intervals were obtained by the area under the curve (AUC) from the specific interval using GraphPad Prism.

    Article Title: Exploring the role of Müller cells-derived exosomes in diabetic retinopathy.
    Article Snippet: Exosomes are nanosized vesicles that have been reported as cargo-delivering vehicles between cells.. Müller cells play a crucial role in the pathogenesis of diabetic retinopathy (DR).. Activated Müller cells in the diabetic retina mediate disruption of barrier integrity and neovascularization.

    Article Title: Aging-regulated PNUTS maintains endothelial barrier function via SEMA3B suppression.
    Article Snippet: .. Endothelial cell impedance and transwell assays After lentiviral transduction and/or transfection, HUVECs were plated in 96-well arrays (96W10idf, Applied Biophysics) at 40,000 cells/well and endothelial cell impedance was measured using the multi-frequency mode for 48 h (ECIS Zθ, Applied Biophysics). ..

    Article Title: Proof-of-concept study: APOE4 brain endothelial cells as a phenotypic compound screen
    Article Snippet: .. After puromycin was removed, brain endothelial cells were washed and grown to confluence in EBM-2 media for 72 h. Brain endothelial confluence was measured via capacitance (frequency of 64 K Hz) and permeability by trans endothelial electrical resistance (TEER, frequency of 10 K Hz) on an ECIS ® ZΘ (Applied Biophysics). ..

    Article Title: PM 10 dysregulates epithelial barrier function in human corneal epithelial cells that is restored by antioxidant SKQ1.
    Article Snippet: .. Cell barrier function The changes in epithelial barrier function caused by PM10 exposure of HCE-2 cells and their rescue by SKQ1 were assessed by measuring normalized transcellular electrical resistance (TER) using an electric cell-substrate impedance sensing [ECIS®Zθ (theta)] instrument (Applied Biophysics Inc. ..

    Transduction:

    Article Title: Aging-regulated PNUTS maintains endothelial barrier function via SEMA3B suppression.
    Article Snippet: .. Endothelial cell impedance and transwell assays After lentiviral transduction and/or transfection, HUVECs were plated in 96-well arrays (96W10idf, Applied Biophysics) at 40,000 cells/well and endothelial cell impedance was measured using the multi-frequency mode for 48 h (ECIS Zθ, Applied Biophysics). ..

    Transfection:

    Article Title: Aging-regulated PNUTS maintains endothelial barrier function via SEMA3B suppression.
    Article Snippet: .. Endothelial cell impedance and transwell assays After lentiviral transduction and/or transfection, HUVECs were plated in 96-well arrays (96W10idf, Applied Biophysics) at 40,000 cells/well and endothelial cell impedance was measured using the multi-frequency mode for 48 h (ECIS Zθ, Applied Biophysics). ..

    other:

    Article Title: Is laccase from medicinal mushroom Cerrena unicolor cytotoxic to colon cancer cell line CT-26?
    Article Snippet: The ECIS (ECIS Zθ, Applied Biophysics, Inc.

    Permeability:

    Article Title: Proof-of-concept study: APOE4 brain endothelial cells as a phenotypic compound screen
    Article Snippet: .. After puromycin was removed, brain endothelial cells were washed and grown to confluence in EBM-2 media for 72 h. Brain endothelial confluence was measured via capacitance (frequency of 64 K Hz) and permeability by trans endothelial electrical resistance (TEER, frequency of 10 K Hz) on an ECIS ® ZΘ (Applied Biophysics). ..



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    A) hCMEC barrier resistance was assessed by <t>ECIS</t> <t>Zθ</t> to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001
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    Image Search Results


    A) hCMEC barrier resistance was assessed by ECIS Zθ to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001

    Journal: bioRxiv

    Article Title: Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models

    doi: 10.64898/2026.03.16.711716

    Figure Lengend Snippet: A) hCMEC barrier resistance was assessed by ECIS Zθ to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001

    Article Snippet: Trans-endothelial electrical resistance was measured using the ECIS ZΘ system (Applied Biophysics).

    Techniques: Western Blot

    CA-VB KO in hCMEC protects from Aβ-induced apoptosis and BBB permeability . A) WB confirmed CA-VB absence in KO cells. B) DNA fragmentation, plotted as fold of change (FOC) of untreated control (ctrl) cells, measured by cell death ELISA. C) Representative IF images of active caspase-3/7 (green) and quantification to the right in CMEC treated with 25µM Q22 for 24 hours. Original magnification: 10x . D) IF of CA-VB KO vs WT hCMEC to detect mitochondrial membrane potential (Mito-tracker, red) as well as cytochrome C (Cyto C, green), in hCMEC challenged with Q22 for 16 hours. Zoom images of the merge signal at the bottom depict altered mitochondrial network with perinuclear mitochondria in Q22-treated hCMEC, but not in CA-VB KO cells. Original magnification:100x E) Mitochondrial H 2 O 2 measured with Amplex Red kit. F) Barrier resistance was measured over time with ECIS-Zθ in WT and CA-VB KO hCMEC treated or not with 10µM Q22. Data represents 3 individual experiments with 2 replicates each, graphed as mean ± SEM. Statistical significance was evaluated by Two-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01 ****P<0.0001

    Journal: bioRxiv

    Article Title: Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models

    doi: 10.64898/2026.03.16.711716

    Figure Lengend Snippet: CA-VB KO in hCMEC protects from Aβ-induced apoptosis and BBB permeability . A) WB confirmed CA-VB absence in KO cells. B) DNA fragmentation, plotted as fold of change (FOC) of untreated control (ctrl) cells, measured by cell death ELISA. C) Representative IF images of active caspase-3/7 (green) and quantification to the right in CMEC treated with 25µM Q22 for 24 hours. Original magnification: 10x . D) IF of CA-VB KO vs WT hCMEC to detect mitochondrial membrane potential (Mito-tracker, red) as well as cytochrome C (Cyto C, green), in hCMEC challenged with Q22 for 16 hours. Zoom images of the merge signal at the bottom depict altered mitochondrial network with perinuclear mitochondria in Q22-treated hCMEC, but not in CA-VB KO cells. Original magnification:100x E) Mitochondrial H 2 O 2 measured with Amplex Red kit. F) Barrier resistance was measured over time with ECIS-Zθ in WT and CA-VB KO hCMEC treated or not with 10µM Q22. Data represents 3 individual experiments with 2 replicates each, graphed as mean ± SEM. Statistical significance was evaluated by Two-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01 ****P<0.0001

    Article Snippet: Trans-endothelial electrical resistance was measured using the ECIS ZΘ system (Applied Biophysics).

    Techniques: Permeability, Control, Enzyme-linked Immunosorbent Assay, Membrane